Friday, October 31, 2008

366 Days, 366 Photos: 10/29/2008

10/29/2008

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Angela made me a 5 foot long corduroy "fake boyfriend" pillow. I actually ordered the stuffing online, so for about a week until it showed up, we kept talking about when my "boyfriend guts" were going to arrive. I occasionally substituted the word "innards" as well.

And damn. Looking at this photo, all I want to do now is go home and snuggle up in my bed.

Thursday, October 30, 2008

Show Review: The Mountain Goats, Kaki King @ Belly Up Tavern, 10/28/2008


Kaki King had just started playing when I arrived at the Belly Up, and I was immediately blown away by her guitar talent and stage presence. Her material was quite a mix - some instrumental pieces, some with just her on stage with her guitar, and some with her full band (drummer, another guitarist, bassist, and keyboardist). The full band arrangements were pretty rocking - her drummer had skills for sure. A lot of the songs had a sort of shoe-gazey atmospheric bent to them, and they were all really beautiful.

Kaki King @ Belly Up Tavern, 10/28/2008

In between songs, she told us a story about walking around in La Jolla last time she was here, holding hands with her girlfriend. And thinking that "hey, we're in southern California, the liberal bastion of hope," so not thinking much of it, really. Then, being La Jolla, a maroon PT Cruiser full of guys pulled up and started harassing them (and yes, the irony of the situation was not lost on her guitarist: "A bunch of guys in a maroon PT Cruiser? How gay!"). So she put in her nod for "No on Prop," so that she could feel safe and secure walking around with her girlfriend. Something that everyone deserves, no?

Kaki King @ Belly Up Tavern, 10/28/2008

On a lighter note, she also asked the crowd what they thought her band should dress up as for Halloween. Someone in the crowd suggested Devo, and they all really seemed to dig that idea. So much so, that her guitarist and drummer launched into "Whip It" on the spot. Pretty humorous. They played quite a long set for an opener, but no one in the crowd was complaining.

Kaki King @ Belly Up Tavern, 10/28/2008

Then came the Mountain Goats. It was a good show, but John was sick and started dragging towards the end. Not that he didn't put a lot of energy into it - we just didn't get multiple encores like the last time I saw them in San Francisco. And the audience had to sing the lyrics to "No Children" during the encore, which actually worked out pretty well.

The Mountain Goats @ Belly Up Tavern, 10/28/2008

Even though John was sick, he was still quite talkative between songs. He introduced "How to Impress a Swamp Creature" by saying that it was about one of those times where it's been a while since you'd "gotten some," so you go visit "someone with whom you used to sleep," even though you know it's a bad idea. But you do it anyway. "This song, it's about the moment where you realize how bad that idea actually was." Yeah. Who HASN'T been there? And yes, I swooned a little at his proper grammar usage on the "with whom you used to sleep" part...

The Mountain Goats @ Belly Up Tavern, 10/28/2008

John's acoustic solo version of "You or Your Memory" gave me chills with its chorus of "St Joseph's baby aspirin. Bartles and Jaymes. And you, or your memory." Darnielle's lyrics are incredibly descriptive and make me think more of reading a short story than listening to a song. And it's odd that so maybe people relate to or find comfort in his songs - so many of them describe hopeless tragic situations. Characters in some of his songs remind me in a way of F. Scott Fitzgerald subjects - beautiful, but for some reason totally bent on destroying one another or even themselves.

The Mountain Goats @ Belly Up Tavern, 10/28/2008

I guess maybe it's the Jerry Springer effect? People turn to these discouraging songs because the songs make them feel better about their own lives? Or the rubberneck effect - you know that something terrible happened, but you want to be a part of it in some way, at least as an observer? Whatever it is, there are somehow few things as thrilling as yelling out the lyrics to "No Children," full of bitterness and hate couched in devotion. It's like telling everyone you were ever angry at exactly what you think of them, but, you know, without the consequences:

"I hope that our few remaining friends
Give up on trying to save us
I hope we come out with a fail-safe plot
To piss off the dumb few that forgave us

I hope the fences we mended
Fall down beneath their own weight
And I hope we hang on past the last exit
I hope it's already too late

And I hope the junkyard a few blocks from here
Someday burns down
And I hope the rising black smoke carries me far away
And I never come back to this town again in my life

I hope I lie
And tell everyone you were a good wife
And I hope you die
I hope we both die

I hope I cut myself shaving tomorrow
I hope it bleeds all day long
Our friends say it's darkest before the sun rises
We're pretty sure they're all wrong

I hope it stays dark forever
I hope the worst isn't over
And I hope you blink before I do
And I hope I never get sober

And I hope when you think of me years down the line
You can't find one good thing to say
And I'd hope that if I found the strength to walk out
You'd stay the hell out of my way

I am drowning
There is no sign of land
You are coming down with me
Hand in unlovable hand

And I hope you die
I hope we both die."

They ended with a rousing rendition of "Michael Myers Resplendent," the song they opened up every set with last tour. And even though people clapped and cheered loudly, we all knew that was it - the house lights were up and the music was on as soon as the band stepped foot off stage. A bummer, but I hope John feels better for the rest of the tour.

366 Days, 366 Photos: 10/28/2008

10/28/2008 - Kaki King @ Belly Up Tavern

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Kaki King's drummer. He was pretty fucking amazing. And I love when bands put their drummers at the front of the stage. Although in this case, it was probably because The Mountain Goats' drumkit was set up behind them. But still. Awesome all around.

Phillies fans, you earned it...


This is the reply you get when you tried to call someone last night at approximately 12AM EST. Someone who lives in Philly. Someone who has lived in Philly all their life except for the 4 years they spent at Penn State.

Yeah.

Congrats, Phillies. And congrats to the fans who stuck out for so many years. I'm always going to be a Pittsburgh fan, but your devotion to your sports teams is...admirable.

Also, I have to agree with this post here.

Wednesday, October 29, 2008

366 Days, 366 Photos: 10/27/2008

10/27/2008

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Another thing I picked up in Fiji. Because turtles are a symbol of good luck over there, and knowing that I was moving to San Diego a week after returning from that trip, I figured I needed all the good luck I could get. Also, I had just come from Australia, where on a dive off the Great Barrier Reef we saw a sea turtle. Pretty fucking rad.

So this guy has been hanging over my bedroom door for the last 4 years. And it's not like everything's been great that entire time, but nothing's been all that terrible either, at least not in retrospect.

I've been hearing a lot of "if it's meant to be, it'll be" lately, and I'm not sure what to make of this. I've never been sure what to make of this. Especially not when said by people who I know don't subscribe to any sort of belief in a higher being.

It doesn't make sense. Life is a random pattern of events. Or so the scientist in me likes to think. But then, yeah, looking through my life, it's pretty easy to see one thing leading to another and shit just working out for the best, even if I didn't see it that way at the time.

Is there something to it? Or do people just feel the need to force a pattern on all the randomness of life, in order to make some sense of it all? So that it's not all entirely meaningless and depressing?

I don't have an answer. This one's up there with "Why do all the atheists I know act more like Christians than, well, the Christians I know? Shouldn't they all just be total assholes if they don't believe a higher being is going to punish them for their sins?" The crossroads of being a non-religious person and an eternal optimist is a really weird place to be. I'm not entirely sure how I got here myself...

366 Days, 366 Photos: 10/26/2008

10/26/2008

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I assisted on a wedding shoot on Sunday, and at the end, they released some butterflies, which was pretty cool.

The wedding was later in the day, thankfully, as I ended up sleeping in until 11AM. I might have slept longer too, except someone called and woke me up. Upon realizing this, he said, "Crap, I just woke you up to ask you a photography question."

My reply was something along the lines of "If you could do this every day, that'd be awesome."

Too bad I think he thought I was joking...

Sunday, October 26, 2008

366 Days, 366 Photos: 10/25/2008

10/25/2008

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Can I just say, that I love having the type of friends who can go from serious conversations about relationships to playing drinking games in less than 30 minutes? Shown here: speed-quarters. Not shown: the recent PhD graduate who said, "How is it that I'm learning to play drinking games AFTER getting my PhD?"

All the cracks you see can be repaired

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The days start to fade out of the frame,
Like they're blurring into someone else's name.
You try your hardest to rewind the tapes,
But you're prepared every day
To make the same mistakes.

Saturday, October 25, 2008

366 Days, 366 Photos: 10/24/2008

10/24/2008

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Fiji was good times.

366 Days, 366 Photos: 10/23/2008

10/23/2008

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Southern California hates my eyes. It's too dry, and the past few days I've had random eye pain. Not okay, especially since I am entirely too dependent on my contact lenses. I have glasses, but I'm really not used to wearing them, so the lack of peripheral vision throws me off. But these eyedrops are awesome, and the past two days my eyes have been a lot better.

366 Days, 366 Photos: 10/22/2008

10/22/2008

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I really like soup. Unfortunately, there are not too many good days for eating soup in San Diego. Am I actually saying I miss the cold? I guess so. It's better for sleeping and snuggling - both activities that I enjoy very much.

I think I'm gonna have to try to get up to Lake Tahoe sometime after Christmas to visit Krystal and get in some decent skiing and cold weather...

Friday, October 24, 2008

366 Days, 366 Photos: 10/21/2008

10/21/2008

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I bought a new belt. Two, actually. Because every single one that I already owned has either fallen apart or is just about to. And I like it when my pants stay up. At least most of the time...

I described my fashion "style" to someone the other day as "indie rock grad student." Meaning that half of my shirts are band t-shirts. Some of which I got for free (local bands) and some that I actually paid for. I'm actually wearing one of three National shirts that I own right now. The other clothes? Of the Old Navy/Target/cheap variety. Because I'm living on a grad student stipend.

Although who am I kidding? If I made more money, I'd just have a lot more band t-shirts. And some sweet camera equipment. Priorities.

What is this motivation crap and where the hell did it come from?

It's weird. The past two weeks I've had a renewed sense of "I need to get my shit together and get the hell out of grad school." To the end that I'm busting my ass to get science done, asked a friend for a copy of his thesis (so I can start thinking about writing my own), and told a committee member that my post-Ph.D. plans do not include doing more scientific research.

More specifically:

I hate optimizing experimental protocols. This is why I have "bad hands" in lab. I'm great at coming up with project ideas, great at figuring out how to approach them experimentally, but when it comes to tweaking the protocols to make them work, I just don't have the patience. It's hard for me to repeat an experiment 8 times over, slightly different each time, to see which way works best. I just want someone to hand me a set of instructions to follow that WORKS. On a side note, this is probably why I'm better at baking then cooking.

But I digress. The point is, I hate optimizing experiments. Yet, I've just mapped out two weeks worth of doing just that. Because once this protocol is ironed out, I will be a hell of a lot closer to being done.

What am I doing, exactly? Trying to purify a protein out of cells, along with any other proteins that happen to be bound to it, and then identify those other proteins. To do this, I've "tagged" this protein with an amino acid sequence that binds to IgG, which can be attached to tiny beads. So you take a bunch of cells, burst (lyse) them open, and incubate the lysate with these beads. Then you centrifuge down the beads (with the protein and whatever's bound to it stuck to them) and wash off whatever isn't stuck to them.

To get my protein off the beads, there is also a specific amino acid sequence engineered after the "tag" that sticks to the beads. That sequence is the recognition sequence for an enzyme that then cuts the protein at that spot. So, theoretically, I add this enzyme to the beads, it does its job and cuts the protein off the beads, and I collect whatever comes off.

The problem with my project is that this enzyme is not working on my protein. I think it is because my protein is folding in such a way as to hide the specific amino acid sequence that the enzyme recognizes. I can't know for sure, because it's still pretty impossible to predict protein structure based on sequence, and I'm no x-ray crystallographer. And it's not really that important in the long run, because I should be able to get my protein off the beads by adding a low concentration of a certain chemical.

And that's the part that needs optimizing. But I'm up for it these days. Because once I have this purification scheme optimized, I should be able to generate a lot of data in a short time. The goal is to purify out my protein (+ binding partners) under different signaling conditions (i.e. after adding different proteins to my cells in culture before I burst them open), and see what comes out with it. The overarching goal is to understand how my protein regulates two different signaling pathways that have two entirely different outcomes.

Okay, I've probably lost everyone at this point. The main idea is - I'm making progress. And willing to work hard at it. I need to be out sometime during next school year. By spring of 2010, at the very latest.

Which is why I'm thinking it might not be a bad idea to start writing up the background section of my thesis, umm, now. Or whenever I happen to have a free moment. Like maybe when I'm home for 10 days over Christmas? That's why I asked P to email me a copy of his thesis. It'll be a lot easier if I have something to look at as a guide. And I can use the background section of a grant I wrote up last year as a start.

So why this renewed sense of motivation? Hmmm. Well, a girl's gotta keep some secrets, right?

And please, someone tell me if the science talk made any sense whatsoever. I'm interested to know.

Thursday, October 23, 2008

Decision Making Skillz

Excerpt from an IM conversation today:

me: brb I have to pee and then set up a PCR reaction
me: tmi but I've been sitting here trying to decide which to do first
me: until time made the decision for me haha

We also talked about the Amish. And driving for 6 hours alone in the car with a strict no bathroom break policy.

And now I'm wondering exactly how many hours of my life were spent driving between State College, PA and Avalon, NJ the summer after sophomore year of college. A LOT. I kind of miss the PA turnpike. And road trips in general. Hmmm.

Tuesday, October 21, 2008

New Frightened Rabbit Live Album out today

Liver! Lung! FR! is out now.

The two times I've seen Frightened Rabbit live, they've totally blown me away. And the first of those times, I had no clue who they were. So that says a lot (another thing that says a lot - they were on a bill with Swim Party and Quasi).

In addition to this, their latest CD, The Midnight Organ Fight, essentially soundtracked my entire trip to Alaska. Hush - I'm allowed to be angsty every once in awhile.

For those who need proof of their awesomeness, here's a live video of "Fast Blood" from their last Casbah show. Apologies for the shakiness at the beginning, my drink was on the stage and I decided I needed to multitask and drink while filming. It was that kind of night:



They're at U-31 on November 5th if you want to see it in person. I will definitely be there.

Monday, October 20, 2008

Show Review: Conor Oberst and the Mystic Valley Band @ Soma, 10/19/2008

Photos: Conor Oberst and the Mystic Valley Band @ Soma

Seriously, Conor. That was kind of awkward.

Conor Oberst and the Mystic Valley Band @ Soma, 10/19/2008

You were drunk. Obviously so. And with reason - I think it was the last night of your tour.

The awkwardness? It's because the rest of us were all sober. Because the show was at Soma. And not everyone has the foresight to drink in their car in the parking lot before the show. Or sometimes the buzz you get from drinking Jameson chased with Sparks wears off a little too soon.

10/19/2008 - Conor Oberst and the Mystic Valley Band @ Soma

Or sometimes the venue is embarrassingly empty. This confused me as well. Either people don't realize that Conor Oberst = that dude from Bright Eyes, or people really didn't want to come to Soma. A little of column A, a little of column B, is my guess. The young folks probably didn't make the connection, and the older folks who did probably didn't want to deal with the all-ages venue.

Conor Oberst and the Mystic Valley Band @ Soma, 10/19/2008

That's the only reasoning I can think of to account for a crowd that was less than a third of what showed up for the last Bright Eyes tour through town.

Conor Oberst and the Mystic Valley Band @ Soma, 10/19/2008

The band sounded great, and as awkwardly drunk as you were, at least it was entertaining. Unfortunately, Soma is not the most acoustically sound venue, and I was getting a lot of lower level noise shoved in my left ear. Tried moving around a bit to find a better spot, but couldn't get rid of the reverberation entirely.

Conor Oberst and the Mystic Valley Band @ Soma, 10/19/2008

I don't mean to say that this wasn't a good show. It just would have been better...elsewhere. The Belly Up, perhaps? I think your recent albums have skewed more towards an older crowd anyway. I like that. I just wish I didn't have to go to one of the worst venues in town to see you play that material live. And I wish I hadn't felt like you were the only drunk person in the entire building.

Conor Oberst and the Mystic Valley Band @ Soma, 10/19/2008

366 Days, 366 Photos: 10/20/2008

10/20/2008

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Another self-portrait. I was feeling pretty lazy today. Wearing glasses because Southern California hates my eyes. My contacts were killing me all day at work today, leading to a headache, leading me to not go see Ian MacKaye speak on campus. Blerg.

366 Days, 366 Photos: 10/19/2008

10/19/2008 - Conor Oberst and the Mystic Valley Band @ Soma

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Felt a little rusty shooting a show, since the last time I shot one was the end of September. Nice to have full run of the pit though. And thanks again to Petro for hooking me up with the photo pass.

Video - The Mountain Goats, "Dance Music" - Sock Puppets



This is just too cute not to share. Stumbled upon it Saturday night.

Sock puppets are awesome, is all I have to say.

Oh, and The Mountain Goats are playing the Belly Up on October 28th. Be there!

366 Days, 366 Photos: 10/18/2008

10/18/2008

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This photo was kinda boring to me, so I mucked with it a bunch in post-processing. Of course. I didn't even really mean for it to be my photo of the day, but I also didn't plan on drinking and hanging out with an acquaintance-turned-friend until 2:30AM. Sometimes these things happen. And any night when I introduce someone to The Mountain Goats is a night to be proud of.

Also, this car reminded me of British Joe's old Mini, which reminded me of rolling up to the Beauty Bar at Halloween two years ago with him dressed up like Austin Powers and me wearing this. It wasn't really planned in any way, but it worked out perfectly. And, for the record, it was the only time I've ever felt like I fit in with the Beauty Bar crowd. When I was wearing a COSTUME.

Sunday, October 19, 2008

366 Days, 366 Photos: 10/17/2008

10/17/2008

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This just makes me giggle. And I needed that Friday night.

366 Days, 366 Photos: 10/16/2008

10/16/2008

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At the pub the other day:
Me: "Why are boys stupid?"
Adam: "Not all boys are stupid."
Me: "ALL boys are stupid SOMETIMES."
...pause. We all kind of nod.
Derek: "Well, then, ALL girls are crazy SOMETIMES."
...another pause. More nods.
Me: "I think we can all live with both of those statements."

366 Days, 366 Photos: 10/15/2008

10/15/2008 - Fireworks

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Fireworks on the USS Midway. I was hired to shoot an event taking place on the aircraft carrier (thanks Andi!), and it was a heck of a lot of fun. So much that I couldn't believe I was getting paid for it. Great people to work with, and a really awesome evening. Hell, any evening that ends with fireworks (and me taking photos of them) is pretty great. A couple more photos (mostly outtakes) here.

Photos: Seattle Holga shots, 06/2008

Photos: Seattle Holga shots

Yeah. So who shoots a roll of film in her Holga in Seattle in JUNE, and then lets it sit in her fridge until OCTOBER? This girl. That's right. There's actually another roll from Alaska still sitting in there right next to the eggs. Maybe I'll get around to developing it today.

These shots came out really well exposed. Seattle was gorgeous the entire time we were there - sunny and warm. And damn if it didn't make me feel right at home right away. Funny how certain places can do that, even if you've never set foot in them before.

Seattle, Holga 04

Seattle, Holga 05

Seattle, Holga 11

Seattle, Holga, 07

Seattle, Holga 02

Quick tip for cleaning up scanned film negatives - to remove dust, use the Photoshop clone stamp tool, but set the mode to "darken." Makes it really easy to get rid of those annoying speckles.

Saturday, October 18, 2008

Photos: Six Flags Magic Mountain

I finally got around to posting more of my Holga and Fisheye photos from Six Flags Magic Mountain a few weekends ago. Here are some of the better ones.

Six Flags Magic Mountain Fisheye 06

Six Flags Magic Mountain Fisheye 14

Six Flags Magic Mountain Fisheye 17

Six Flags Magic Mountain Fisheye 20

Six Flags Magic Mountain Holga 01

Six Flags Magic Mountain Holga 02

Six Flags Magic Mountain Holga 03

Six Flags Magic Mountain Holga 06

Wednesday, October 15, 2008

Some Perspective

At lunch today -

Me: "Why are all my friends graduating?"

P: "Because your friends are all old 7th years."

Crap. I just started my 5th year. I'm going to shoot myself in the face if I'm still sitting at this desk without a Ph.D. in two years...

Oh yeah, there's my motivation. Welcome back.

Here's something that actually worked yesterday:

And just to even things out, here's something that didn't. Because science is a bitch like that:

366 Days, 366 Photos: 10/14/2008

10/14/2008

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I literally could not get off my couch last night. After working 21 hours in two days, I had no desire to go down to the Casbah. Or try hard to take a cool photo. So this is what you get.

Monday, October 13, 2008

366 Days, 366 Photos: 10/13/2008

10/13/2008

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Hold ourselves together, with our arms around the stereo, for hours.
Love the sentiment in that single lyric. That is all.

366 Days, 366 Photos: 10/12/2008

10/12/2008

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Finally managed to get a decent photo of Bailey. Of course not the one where he was sleeping with his tail over his nose, but this'll do. He tried to make out with me at least 5 times that night (and I didn't even have beer breath!) and literally pawed my boobs. He's a fresh one, he is.

366 Days, 366 Photos: 10/11/2008

10/11/2008

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I really have this thing for signs painted onto brick work. I think it reminds me of old, poor East Coast cities. Or I'm just romanticizing. I dunno. Love the way the paint calls attention to the texture of the brick. Or something like that.